Response surface modelling of the production of structured lipids from soybean oil using Rhizomucor miehei lipase
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چکیده
منابع مشابه
Structure-Guided Modification of Rhizomucor miehei Lipase for Production of Structured Lipids
To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87Ile/Asp91Val, His108Leu/Lys109Ile, Asp256Ile/His257Leu, and His108Leu/Lys109Ile/Asp256Ile/His257L...
متن کاملImmobilization of Rhizomucor miehei Lipase on High Density Polyethylene
Immobilization of Lipase produced from Rhizomucor miehei on HDPE fine powder was investigated. As compared to an aqueous system, immobilization in a non-aquous organic medium such as n-hexane was not successful and caused enzyme denaturation. Prewetting the support with ethanol increased the immobilized protein and enzyme activity as much as 31% and 34%, respectively. The maximum immobilized a...
متن کاملProduction of MLM-Type structured lipids from fish oil catalyzed by Thermomyces lanuginosus lipase
Background Lipases are powerful tools for the syntheses of structured lipids (SL) which are triacylglycerols (TAG) having particular fatty acid residues at specific positions. The production of TAG with medium chain fatty acids (MCFA), in positions sn-1 and sn-3, and a long-chain fatty acid (LCFA), in the internal position, has recently increased due to its nutritional interest in applications ...
متن کاملstructured lipids produced through lipase-catalyzed acidolysis of canola oil
enzymatic acidolysis of canola oil through caprylic acid was investigated to produce certain medium chain ttriacyliglycerol (tag) structured lipids (sls). lipozyme tl im, an sn-1,3 specific thermomyces lanuginosa lipase, and novozym 435, a non-specific candida antarctica lipase, were utilized as the biocatalysts in a batch reactor. reaction conditions were designed according to taguchi’s approa...
متن کاملEnantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones.
Enantioselectivity of enzyme catalysis is often rationalized via active site models. These models are constructed on the basis of comparing the enantiomeric excess of product observed in a series of reactions which are conducted with a range of homologous substrates, typically carrying various side chain substitutions. Surprisingly the practical application of these simple but informative 'pock...
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ژورنال
عنوان ژورنال: Food Chemistry
سال: 2011
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2010.12.072